Sequencing Services

Sequencing Services

 

MR DNA is a full service next generation sequencing service provider.   If you do not see the application of interest in our sequencing applications (linked to the left) just contact us via phone or email and we will definitely help you with any type of project.

 

examples of our popular sequencing programs

AMPLICON SEQUENCING (bTEFAP® services)

 

examples of amplicon sequencing includes microbiome assays such as 16s ribosomal sequencing,  ITS region for fungi,  18s for general eukaryotes,  functional genes such as nirS, nifH, dsr, rpoB, or ANY other target or custom amplicon.

 

we have several platforms that can be used for amplicons:

 

 

 

 

 

**Ion S5 XL (Ion Torrent) amplicons

All Ion S5 XL projects are being migrated to the Illumina MiSeq and Illumina NovaSeq Sequencing Platforms

 

All economy sequencing projects previously completed using the Ion S5 will now be completed using the Illumina MiSeq and NovaSeq at no additional cost.

 

  • The same 515F-806R and ITS1-2 primers will continue to be made available for use on all Illumina sequencing platforms
  • Contact us to discuss additional Illumina specific primer sets and pricing options

 

300bp 515F bacterial + archaeal or ITS1-2 fungal diversity Ion S5 assays with nominal 15-20,000 reads/assay for $60/assay (any size project)

 

>400bp bacterial, archaeal, or ITS fungal diversity assays with nominal 15-20,000 reads/assay starting at $85/assay and continues to follow the Illumina MiSeq pricing scale below

 

Illumina MiSeq:

 

2x300bp PE illumina 20,000 sequence diversity assays starting $85/assay 1-50 assays (note: for projects < 10 assays per library, a $100 library fee is added),  $80 for 50-100 assays, 75 for 100-150 assays, $70 for 150-300, $65 for > 300 assays.

 

PacBio SEQUEL:

 

Long read amplicons (e.g. near full 16s ~1400bp, ITS1-4, 18s ~1700bp or other custom amplicon diversity (700-2500bp) ( usually minimum 10 samples or $150 library fee is added )

 

Academic and Government Pricing

$90 per sample  for 5,000 sequences per assay,

$125 per sample for 10,000 sequences per assay

$200 per sample for 20,000 sequences per assay

$340 per sample for 40,000 sequences per assay

 

 

**Custom assays from 700bp - 3000bp or larger of course have a barcoding fee that is typically $15/barcode

 

 

 

Here are examples of Genome and metagenome programs.  Note that we do have bulk discounts for large projects..  these examples are for small projects e.g.  < 5 samples

 

*** Genomes or Metagenome shotgun DNA :

- 2x150bp 10-20 million paired sequences per sample = $400 (data only)

-2x300bp 1-2 million sequences = $600  (data only)

-2x250bp 10 million sequences = $650(data only)

-2x250bp  4 million sequences = $450(data only)

 

 

NEW:  gap closure sequencing long read scaffolding = $800/sample

Data assembly with optimization = $150

-----we have all kinds of bioinformatics services for shotgun platforms  just ask ;-)

 

Here are examples for sequencing RNA samples such as RNAseq and metatranscriptomes..  These are small project prices but we do have bulk discounts for large projects.

 

***Transcriptome Metatranscriptome  shotgun RNAseq:

- 2x150bp 10-20 million paired sequences per sample = $460 (data only)

- 2x300bp 1-2 million sequences = $700  (data only)

- 2x250bp 10 million sequences = $710 (data only)

- 2x250bp 4 million sequences = $510 (data only)

--- Ribosomal depletion if needed for metatranscriptomes or bacterial transcriptomes= $300

 

DNA Extraction / RNA Extraction Price:

--DNA Extraction starting at $25 / sample (depending on service requested and sample type submitted)

--RNA Extraction starting between $30 - $70 / sample (depending  on service requested and sample type submitted)

Sequencing Platform Read Length /Assay Length Pricing
Ion S5 XL** 300bp 515F bacterial + archaeal or ITS1-2 fungal diversity For nominal 15-20,000 reads/assay:
$60/assay (any size project)
Ion S5 XL (EXT)** >500bp bacterial, archaeal, Same Pricing as Illumina MiSeq
or ITS fungal diversity assays (No Additional Library Fee)
    20,000 sequence diversity assays starting at:
$60 /assay (>150 assays)
$65/assay (100-150 assays)
Illumina MiSeq 2x300 PE $70/assay (50-100 assays)
$75/assay (20-50 assays)
$80/assay (1-20 assays)
For projects < 10 samples a $100 library fee is added
    Low Coverage (< 500 Seqs): $50/assay
~5,000 Seqs: $90/assay
PacBio Sequel Long Read Amplicon Sequencing ~10,000: $125/assay
(16s, 18s, ITS, and Custom) ~20,000: $200/assay
~40,000: $340/assay
Custom Coverage available; Contact us for pricing
For projects < 10 samples a $150 library fee is added & $20/sample PCR replication fee

Communal Genomes or Metagenome:

 

Our new program provides $400 bacterial genomes and metagenomes. This program provides roughly 10 million paired sequences per sample. It is important to note the turnaround for these vary from a few weeks to a few months, therefore this program is only recommended for projects not on a strict deadline.

 

Microbiome Analyses:

 

A free comprehensive taxonomic analysis comes with any 16s, ITS, and 18s data (We are also able to provide analyses for several functional genes as well). Included is a full analysis pipeline comprised of denoising, chimera check, low quality sequence cleaning or removal, clustering, otus generation, taxonomic assignment etc.  Our pipeline generates about 12 different analysis files evaluating the taxonomic data at each taxonomic level:  genus, species, family, class, order, and phylum.  Additionally, OTU files are provided with lineage and identity information as well as other statistical values. To aid in navigating all of the analyses and raw data, a readme help file is provided.

 

For an additional $10/sample (to recover cost only) we perform alpha and beta diversity, rarefaction, unifrac PCoA etc. These analyses are provided raw, but a standard readme help file is provided, making it fairly easy to work through this extensive amount of data.

 

We also offer advanced analysis and report generation. Similar to a research manuscript, the report will contain methods, biostatistics, figures, tables, legends, results section, and findings overview (if relevant).  The cost for advanced analysis and report generation begins at $500 and may increase depending on the size and complexity of the experiment.  Please detail fully your experimental design and the variable information for your samples to give us the ability to accurately quote these costs.  All report generation and other bioinformatics are billed based upon the time required.  We are fast and always make the cost a great value!

 

THE FOLLOWING OPTIONS are also available for targeted amplicon sequencing on each of our platforms:

 

archaea

nifH

HIV

MHC

fungi

protozoa

AND MUCH MORE!

 

 

Our analysis tools are mature for Illumina, Ion PGM / S5, and PacBio Sequel.  For functional genes some setup costs may be necessary for analysis to create a taxonomic database.

 

MRDNA Bacteria Sequencing Contact Number
Sequencing Platform Read Length /Assay Length Pricing
Ion S5 XL 300bp 515F bacterial + archaeal or ITS1-2 fungal diversity For nominal 15-20,000 reads/assay:
$60/assay (any size project)
Ion S5 XL (Ext) >400bp bacterial, archaeal, Same Pricing as Illumina MiSeq
or ITS fungal diversity assays (No Additional Library Fee)
Illumina MiSeq 2x300 PE 20,000 sequence diversity assays starting at:
$65 /assay (>300 assays)
$70/assay (150-300 assays)
$75/assay (100-150 assays)
$80/assay (50-100 assays)
$85/assay (1-50 assays)
For projects < 10 samples a $100 library fee is added
PacBio Sequel Long Read Amplicon Sequencing (16s, 18s, ITS, and Custom) Low Coverage (< 500 Seqs): $50/assay
~5,000 Seqs): $90/assay
~10,000: $125/assay
~20,000: $200/assay
~40,000: $340/assay
Custom Coverage available; Contact us for more information